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Bioss collagen i
Collagen I, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human pro collagen i alpha 1 col1a1 duoset elisa  (R&D Systems)
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A Immunofluorescence staining of the cell cytoplasm using Cell Mask (green) and Phalloidin (red). B , C Quantification of cell area and aspect ratio. Gene expression analysis of D ACTA2 , E MYLK , and F MYH11 via qRT-PCR. G Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers H α-SMA, I FAP. Gene expression analysis of J COL3A1 and K <t>COL1A1</t> via qRT-PCR. L Quantification of secreted collagen I using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01,*** p < 0.001 and **** p < 0.0001 compared to the control and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared to TGF-β 1 .
Human Pro Collagen I Alpha 1 Col1a1 Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human pro collagen i alpha 1 quantikine elisa kit  (R&D Systems)
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A Immunofluorescence staining of the cell cytoplasm using Cell Mask (green) and Phalloidin (red). B , C Quantification of cell area and aspect ratio. Gene expression analysis of D ACTA2 , E MYLK , and F MYH11 via qRT-PCR. G Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers H α-SMA, I FAP. Gene expression analysis of J COL3A1 and K <t>COL1A1</t> via qRT-PCR. L Quantification of secreted collagen I using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01,*** p < 0.001 and **** p < 0.0001 compared to the control and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared to TGF-β 1 .
Human Pro Collagen I Alpha 1 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagen type i alpha 1 col1a1  (R&D Systems)
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A Immunofluorescence staining of the cell cytoplasm using Cell Mask (green) and Phalloidin (red). B , C Quantification of cell area and aspect ratio. Gene expression analysis of D ACTA2 , E MYLK , and F MYH11 via qRT-PCR. G Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers H α-SMA, I FAP. Gene expression analysis of J COL3A1 and K <t>COL1A1</t> via qRT-PCR. L Quantification of secreted collagen I using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01,*** p < 0.001 and **** p < 0.0001 compared to the control and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared to TGF-β 1 .
Collagen Type I Alpha 1 Col1a1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human pro collagen i alpha 1 duoset elisa kit  (R&D Systems)
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R&D Systems human pro collagen i alpha 1 duoset elisa kit
A Immunofluorescence staining of the cell cytoplasm using Cell Mask (green) and Phalloidin (red). B , C Quantification of cell area and aspect ratio. Gene expression analysis of D ACTA2 , E MYLK , and F MYH11 via qRT-PCR. G Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers H α-SMA, I FAP. Gene expression analysis of J COL3A1 and K <t>COL1A1</t> via qRT-PCR. L Quantification of secreted collagen I using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01,*** p < 0.001 and **** p < 0.0001 compared to the control and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared to TGF-β 1 .
Human Pro Collagen I Alpha 1 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human pro collagen 1a1 elisa kit  (R&D Systems)
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A Immunofluorescence staining of the cell cytoplasm using Cell Mask (green) and Phalloidin (red). B , C Quantification of cell area and aspect ratio. Gene expression analysis of D ACTA2 , E MYLK , and F MYH11 via qRT-PCR. G Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers H α-SMA, I FAP. Gene expression analysis of J COL3A1 and K <t>COL1A1</t> via qRT-PCR. L Quantification of secreted collagen I using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01,*** p < 0.001 and **** p < 0.0001 compared to the control and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared to TGF-β 1 .
Human Pro Collagen 1a1 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human procollagen iα1 duoset elisa kit  (R&D Systems)
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R&D Systems human procollagen iα1 duoset elisa kit
A Immunofluorescence staining of the cell cytoplasm using Cell Mask (green) and Phalloidin (red). B , C Quantification of cell area and aspect ratio. Gene expression analysis of D ACTA2 , E MYLK , and F MYH11 via qRT-PCR. G Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers H α-SMA, I FAP. Gene expression analysis of J COL3A1 and K <t>COL1A1</t> via qRT-PCR. L Quantification of secreted collagen I using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01,*** p < 0.001 and **** p < 0.0001 compared to the control and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared to TGF-β 1 .
Human Procollagen Iα1 Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human procollagen iα1 duoset elisa kit/product/R&D Systems
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type i collagen  (Bioss)
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A Immunofluorescence staining of the cell cytoplasm using Cell Mask (green) and Phalloidin (red). B , C Quantification of cell area and aspect ratio. Gene expression analysis of D ACTA2 , E MYLK , and F MYH11 via qRT-PCR. G Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers H α-SMA, I FAP. Gene expression analysis of J COL3A1 and K <t>COL1A1</t> via qRT-PCR. L Quantification of secreted collagen I using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01,*** p < 0.001 and **** p < 0.0001 compared to the control and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared to TGF-β 1 .
Type I Collagen, supplied by Bioss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pro collagen 1 alpha 1  (R&D Systems)
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A Immunofluorescence staining of the cell cytoplasm using Cell Mask (green) and Phalloidin (red). B , C Quantification of cell area and aspect ratio. Gene expression analysis of D ACTA2 , E MYLK , and F MYH11 via qRT-PCR. G Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers H α-SMA, I FAP. Gene expression analysis of J COL3A1 and K <t>COL1A1</t> via qRT-PCR. L Quantification of secreted collagen I using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01,*** p < 0.001 and **** p < 0.0001 compared to the control and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared to TGF-β 1 .
Pro Collagen 1 Alpha 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A Immunofluorescence staining of the cell cytoplasm using Cell Mask (green) and Phalloidin (red). B , C Quantification of cell area and aspect ratio. Gene expression analysis of D ACTA2 , E MYLK , and F MYH11 via qRT-PCR. G Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers H α-SMA, I FAP. Gene expression analysis of J COL3A1 and K COL1A1 via qRT-PCR. L Quantification of secreted collagen I using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01,*** p < 0.001 and **** p < 0.0001 compared to the control and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared to TGF-β 1 .

Journal: npj Biomedical Innovations

Article Title: Developing a protocol to counteract spontaneous in vitro activation of intestinal fibroblasts using design of experiments

doi: 10.1038/s44385-026-00079-5

Figure Lengend Snippet: A Immunofluorescence staining of the cell cytoplasm using Cell Mask (green) and Phalloidin (red). B , C Quantification of cell area and aspect ratio. Gene expression analysis of D ACTA2 , E MYLK , and F MYH11 via qRT-PCR. G Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers H α-SMA, I FAP. Gene expression analysis of J COL3A1 and K COL1A1 via qRT-PCR. L Quantification of secreted collagen I using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01,*** p < 0.001 and **** p < 0.0001 compared to the control and ## p < 0.01, ### p < 0.001, and #### p < 0.0001 compared to TGF-β 1 .

Article Snippet: Fibronectin and collagen secretion were quantified using commercially available Human Fibronectin (FN) ELISA Kit (RayBiotech, product # ELH-FN1-1) and Human Pro-Collagen I alpha 1 (COL1A1) DuoSet ELISA (R&D Systems, product # DY6220-05) following the manufacturer’s instructions.

Techniques: Immunofluorescence, Staining, Gene Expression, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Control

A Quantification of cell viability. Gene expression analysis of B ACTA2 , C MYLK , and D MYH11 via qRT-PCR. E Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers F α-SMA and G FAP. Gene expression analysis of H COL3A1 and I COL1A1 via qRT-PCR. Quantification of ECM protein secretion for J collagen I and K fibronectin using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 compared to the control and # p < 0.05, ## p < 0.01, #### p < 0.0001 compared to TGF β 1 -treated group, for comparisons shown.

Journal: npj Biomedical Innovations

Article Title: Developing a protocol to counteract spontaneous in vitro activation of intestinal fibroblasts using design of experiments

doi: 10.1038/s44385-026-00079-5

Figure Lengend Snippet: A Quantification of cell viability. Gene expression analysis of B ACTA2 , C MYLK , and D MYH11 via qRT-PCR. E Immunofluorescence staining of α-SMA (red) and FAP (green), with cell nuclei counterstained with DAPI (blue). Quantification of the staining intensity of the myofibroblastic protein markers F α-SMA and G FAP. Gene expression analysis of H COL3A1 and I COL1A1 via qRT-PCR. Quantification of ECM protein secretion for J collagen I and K fibronectin using ELISA. Data are presented as mean ± SD ( n = 3–5 replicates). Scale bar indicates 100 μm. One-way ANOVA followed by Tukey’s multiple comparisons test. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 compared to the control and # p < 0.05, ## p < 0.01, #### p < 0.0001 compared to TGF β 1 -treated group, for comparisons shown.

Article Snippet: Fibronectin and collagen secretion were quantified using commercially available Human Fibronectin (FN) ELISA Kit (RayBiotech, product # ELH-FN1-1) and Human Pro-Collagen I alpha 1 (COL1A1) DuoSet ELISA (R&D Systems, product # DY6220-05) following the manufacturer’s instructions.

Techniques: Gene Expression, Quantitative RT-PCR, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Control